{"id":9896,"date":"2020-12-01T10:55:14","date_gmt":"2020-12-01T15:55:14","guid":{"rendered":"http:\/\/132.236.156.160\/cuccap\/?p=9896"},"modified":"2022-04-01T16:07:08","modified_gmt":"2022-04-01T20:07:08","slug":"cuccap-researchers-develop-genetic-tools-and-diagnostic-guide-for-cucurbit-downy-mildew","status":"publish","type":"post","link":"http:\/\/132.236.156.160\/cuccap\/2020\/12\/01\/cuccap-researchers-develop-genetic-tools-and-diagnostic-guide-for-cucurbit-downy-mildew\/","title":{"rendered":"CucCAP researchers develop genetic tools and diagnostic guide for cucurbit downy mildew"},"content":{"rendered":"<p><span style=\"font-weight: 400\">Cucurbit downy mildew (CDM)\u00a0 is a devastating disease affecting economically important crops such as cantaloupe, cucumber, squash, pumpkin, and watermelon. Plant pathologists from the CucCAP disease management team published two articles focusing on CDM.<\/span><\/p>\n<hr \/>\n<p><span style=\"font-weight: 400\">In the first article, plant pathologists at North Carolina State University determined that the causal pathogen of Cucurbit downy mildew, <\/span><i><span style=\"font-weight: 400\">Pseudoperonospora cubensis,<\/span><\/i><span style=\"font-weight: 400\"> has two genetically distinct host-adapted clades and also found that wild cucurbits can serve as reservoirs for this pathogen. Clade 1 isolates more frequently infect squash, pumpkin, and watermelon while clade 2 impacts cucumber and cantaloupe. They also found that evidence of recombination in clade 1 isolates but not clade 2 isolates. While previous research had shown evidence of two subpopulations within the pathogen, none were able to identify the main factors underlying those populations due to sampling limitations. Using a population genetics approach, the researchers applied a robust and standardized sampling strategy to investigate <\/span><i><span style=\"font-weight: 400\">P. cubensis<\/span><\/i><span style=\"font-weight: 400\"> populations that infect cucurbit hosts. Moving forward, plant pathologists can provide crop-specific recommendations.<\/span><\/p>\n<p><span style=\"font-weight: 400\">Read the article: <\/span><span style=\"font-weight: 400\"><br \/>\n<\/span><\/p>\n<ul>\n<li><strong><a href=\"https:\/\/apsjournals.apsnet.org\/doi\/10.1094\/PHYTO-01-20-0009-R\" target=\"_blank\" rel=\"noopener noreferrer\">Population Analyses Reveal Two Host-Adapted Clades of <em>Pseudoperonospora cubensis<\/em>, the Causal Agent of Cucurbit Downy Mildew, on Commercial and Wild Cucurbits<\/a><br \/>\n<\/strong>by: E. C. Wallace, K. N. D\u2019Arcangelo, and L. M. Quesada-Ocampo<em><br \/>\nPhytopathology<br \/>\n<\/em>Published Online:<span class=\"epub-section__date\">7 Jul 2020<br \/>\n<\/span>DOI:\u00a0<a class=\"epub-section__doi__text\" href=\"https:\/\/doi.org\/10.1094\/PHYTO-01-20-0009-R\" target=\"_blank\" rel=\"noopener noreferrer\">10.1094\/PHYTO-01-20-0009-R<\/a><\/li>\n<\/ul>\n<p><a class=\"btn btn-default btn-md\" href=\"https:\/\/apsjournals.apsnet.org\/doi\/pdf\/10.1094\/PHYTO-01-20-0009-R\" target=\"_blank\" rel=\"noopener noreferrer\">View the PDF<\/a><\/p>\n<hr \/>\n<div style=\"width: 210px\" class=\"wp-caption alignright\"><img decoding=\"async\" loading=\"lazy\" class=\"size-thumbnail\" src=\"https:\/\/media.eurekalert.org\/multimedia_prod\/pub\/rel\/246579_rel.jpg\" alt=\"symptoms on leaf\" width=\"200\" height=\"150\" \/><p class=\"wp-caption-text\">Cucurbit Downy Mildew on Cucumber. Credit Andres Salcedo, Mary Hausbeck, Stacey Pigg, and Lina M. Quesada-Ocampo<\/p><\/div>\n<p><span style=\"font-weight: 400\">Cucurbit downy mildew is rapid, infectious, and hard-to-diagnose. Accurate and early diagnosis is critical for timely disease management. Plant pathologists from North Carolina State University and Michigan State University developed a guide for beginners and experts with information about the causal agent of cucurbit downy mildew and multiple alternatives for it\u2019s diagnosis and handling. As cucurbit downy mildew is caused by a pathogen that cannot be cultured using standard microbiological methods, this guide provides well-documented written and graphic material to help with diagnosis based on symptoms and the presence of the pathogen in the plant. The guide also covers diagnosis methods based on DNA testing and microscopy as well as methodologies for isolation, damage evaluation, multiplication, and storage under laboratory conditions.<\/span><\/p>\n<p><span style=\"font-weight: 400\">The ability to quickly identify cucurbit downy mildew is crucial for cost-effective chemical or cultural control strategies. To help those who are trying to assess the efficacy of new disease management products, this guide also provides protocols for pathogen handling and evaluation. Parts of the guide are also being used to develop extension training videos to further inform a broader audience about cucurbit downy mildew.<\/span><\/p>\n<p>Read the Article:<\/p>\n<ul>\n<li><strong><a href=\"https:\/\/apsjournals.apsnet.org\/doi\/full\/10.1094\/PHP-12-19-0095-DG\" target=\"_blank\" rel=\"noopener noreferrer\">Diagnostic Guide for Cucurbit Downy Mildew<\/a><br \/>\n<\/strong>by: Andres Salcedo, Mary Hausbeck, Stacey Pigg, and Lina M. Quesada-Ocampo<em><br \/>\nPlant Health Progress<br \/>\n<\/em><span class=\"epub-section__item\"><span class=\"epub-section__state\">Published Online:<\/span><span class=\"epub-section__date\">14 May 2020<br \/>\nDOI: <\/span><\/span><span class=\"epub-section__item\"><a class=\"epub-section__doi__text\" href=\"https:\/\/doi.org\/10.1094\/PHP-12-19-0095-DG\" target=\"_blank\" rel=\"noopener noreferrer\">10.1094\/PHP-12-19-0095-DG<\/a><\/span><\/li>\n<\/ul>\n<p><a class=\"btn btn-default btn-md\" href=\"https:\/\/www.eurekalert.org\/pub_releases\/2020-10\/aps-ucd102120.php\" target=\"_blank\" rel=\"noopener noreferrer\">Read the news release<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Plant pathologists from the CucCAP disease management team published two articles focusing on CDM: &#8220;Population Analyses Reveal Two Host-Adapted Clades of <em>Pseudoperonospora cubensis<\/em>, the Causal Agent of Cucurbit Downy Mildew, on Commercial and Wild Cucurbits&#8221; &amp; &#8220;Diagnostic Guide for Cucurbit Downy Mildew&#8221;<\/p>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"quote","meta":{"footnotes":""},"categories":[269,241,419],"tags":[399,472,463],"_links":{"self":[{"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/posts\/9896"}],"collection":[{"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/comments?post=9896"}],"version-history":[{"count":25,"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/posts\/9896\/revisions"}],"predecessor-version":[{"id":12437,"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/posts\/9896\/revisions\/12437"}],"wp:attachment":[{"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/media?parent=9896"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/categories?post=9896"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/tags?post=9896"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}