{"id":14298,"date":"2023-06-10T14:59:12","date_gmt":"2023-06-10T18:59:12","guid":{"rendered":"http:\/\/132.236.156.160\/cuccap\/?p=14298"},"modified":"2024-08-07T20:44:08","modified_gmt":"2024-08-08T00:44:08","slug":"melon-team-2023-progress-report","status":"publish","type":"post","link":"http:\/\/132.236.156.160\/cuccap\/2023\/06\/10\/melon-team-2023-progress-report\/","title":{"rendered":"Melon Team | 2023 Progress Report"},"content":{"rendered":"<p>View the <strong><a href=\"http:\/\/132.236.156.160\/cuccap\/wp-content\/uploads\/sites\/6\/2023\/06\/CucCAP.compiledrep.2023.pdf#page=35\" target=\"_blank\" rel=\"noopener\">2023 CucCAP Melon Team Report<\/a> in pages 35-48 of the PDF version of this report<\/strong><\/p>\n<h2>Team members:<\/h2>\n<ul>\n<li>Jim McCreight (USDA, ARS)<\/li>\n<li>Shaker Kousik (USDA, ARS)<\/li>\n<li>Pat Wechter (USDA, ARS)<\/li>\n<li>Bill Wintermantel (USDA, ARS)<\/li>\n<\/ul>\n<h3>CucCAP Affiliated Postdocs and Graduate Students<\/h3>\n<ul>\n<li>Shaonpius Mondal, USDA-ARS, Salinas (McCreight, Wintermantel)<\/li>\n<li>Venkata Rao Ganaparthi- PhD Candidate (Wechter &amp; Branham)<\/li>\n<\/ul>\n<h2>Map and develop markers for disease resistance<\/h2>\n<h3>QTL mapping of resistances<\/h3>\n<ul>\n<li>Powdery mildew, Ph, Q, F. Completed QTL mapping in the MR-1xAY RIL population and narrowed the QTL intervals by adding KASP markers to the GBS data. Developed KASP markers for two major QTL and validated them in a set of unrelated cultivars population.<\/li>\n<li>Fusarium, Ph, Q, F. Completed QTL mapping in the MR-1xAY RIL population and narrowed the QTL intervals by adding KASP markers to the GBS data. Developed KASP markers for the single major QTL for races 1 and 2 and validated them in a set of unrelated cultivars. population.<\/li>\n<li>Downy mildew, Ph, Q, F. Completed QTL mapping in the MR-1xAY RIL population and narrowed the QTL intervals by adding KASP markers to the GBS data. Developed KASP markers for the major QTL. Manuscript accepted in <em>Theoretical and Applied Genetics<\/em>.<\/li>\n<li>Powdery mildew: The F2:3 PI 313970 x Top Mark developed in CA will be planted in a greenhouse at Charleston in November to evaluate for resistance to powdery mildew.<\/li>\n<li>downy mildew<\/li>\n<li>CYSDV: Two QTL for resistance in were found in F2:3 PI 313970 x Top Mark, on chromosomes 3 and 5. The QTL on chromosome 5 was observed in naturally infected field tests in 2018 and 2019, and explained 16 % and 35 % of the variation in CYSDV titer, respectively. The QTL on Chromosome 3 explained 20 % of virus titer variation in 2018 but was undetected in 2019 (Tamang et al. 2021). Single gene recessive, Mendelian resistance was previously reported in PI 313970 (McCreight and Wintermantel, 2011).<\/li>\n<\/ul>\n<p>Eight F2:3 lines with low virus titer as determined by RT-qPCR (Mondal et al. 2023) resembled PI 313970 for the two flanking markers, which can, therefore, be utilized in marker assisted breeding of CYSDV-resistant melons. The eight F2:3 lines were evaluated to date for resistance reaction in a controlled inoculation, growth chamber test. The lines segregated for resistance reactions and flanking markers. Some of the high-titer plants (low CT value) were frequently asymptomatic or exhibited mild yellowing. Low-titer plants with one or both PI 313970 markers and asymptomatic\/mild symptoms been advanced to next generation.<\/p>\n<h3>Marker development and verification<\/h3>\n<ul>\n<li>Powdery mildew, M, V. KASP markers for powdery mildew race 1 resistance were developed in the MR-1xAY RIL population and were validated with a set of commercial cultivars.<\/li>\n<li>Fusarium, M, V. KASP markers for Fusarium wilt races 1 and 2 resistance were developed in the MR-1xAY RIL population and were validated with a set of commercial cultivars.<\/li>\n<li>Downy mildew, M. KASP markers for Fusarium wilt races 1 and 2 resistance were developed in the MR-1xAY RIL population. Manuscript accepted in Theoretical and Applied Genetics.<\/li>\n<li>CYSDV: see above<\/li>\n<\/ul>\n<h3>Introgress, pyramid\/stack resistances into advanced breeding lines<br \/>\nMelon<\/h3>\n<ul>\n<li>Fusarium wilt races 1 and 2\/Powdery mildew race 1, B,I,A. Using KASP markers developed under this project and a backcross\/ phenotype\/genotype strategy, we are currently making seed for BC2F3 populations from the three-way cross of [Top Mark x Majik Melon (resistance donor) x Charentais]. Selections have been made in the early phases of the project for a Charentais-type line and a Top Mark-type line with subsequent backcrosses to either Charentais or to Top Mark. Current lines have high brix, firm flesh, powdery mildew race 1 resistance, sulphur tolerance, and Fusarium race 1 and 2 resistance.<\/li>\n<li>CYSDV: see 2.1.2 above<\/li>\n<\/ul>\n<h4>Figure 1. Development of varieties resistant to multiple diseases in the PDF version of this report.<\/h4>\n<p>View Figure 1. on page 36 of the PDF version of the <strong><a href=\"http:\/\/132.236.156.160\/cuccap\/wp-content\/uploads\/sites\/6\/2023\/06\/CucCAP.compiledrep.2023.pdf#page=36\" target=\"_blank\" rel=\"noopener\">2023 CucCAP Melon Team Report<\/a><\/strong>.<\/p>\n<h3>Literature Cited<\/h3>\n<ul>\n<li>McCreight, J.D. and W.M. Wintermantel. 2011. Genetic resistance in melon PI 313970 to Cucurbit yellow stunting disorder virus. HortScience 46:1582\u20131587.<\/li>\n<li>Mondal, S., L. Jenkins Hladky, and W.M. Wintermantel. 2023. Differential seasonal prevalence of yellowing viruses infecting melon crops in southern California and Arizona determined by multiplex RT-PCR and RT-qPCR. Plant Dis. DOI: <a href=\"https:\/\/doi.org\/10.1094\/PDIS-06-22-1512-RE\" target=\"_blank\" rel=\"noopener\">10.1094\/PDIS-06-22-1512-RE<\/a><\/li>\n<li>Tamang, P., K. Ando, W.M. Wintermantel, and J.D. McCreight. 2021. QTL mapping of Cucurbit yellow stunting disorder virus resistance in melon accession PI 313970. HortScience 56:424\u2013430. DOI: <a href=\"https:\/\/doi.org\/10.21273\/HORTSCI15495-20\" target=\"_blank\" rel=\"noopener\">10.21273\/HORTSCI15495-20<\/a>.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>View the 2023 CucCAP Melon Team Report in pages 35-48 of the PDF version of this report<\/p>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"quote","meta":{"footnotes":""},"categories":[265],"tags":[541,461,335,572,323],"_links":{"self":[{"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/posts\/14298"}],"collection":[{"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/comments?post=14298"}],"version-history":[{"count":7,"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/posts\/14298\/revisions"}],"predecessor-version":[{"id":16743,"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/posts\/14298\/revisions\/16743"}],"wp:attachment":[{"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/media?parent=14298"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/categories?post=14298"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/tags?post=14298"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}