{"id":11888,"date":"2022-01-05T14:20:50","date_gmt":"2022-01-05T19:20:50","guid":{"rendered":"http:\/\/132.236.156.160\/cuccap\/?p=11888"},"modified":"2022-04-01T16:12:34","modified_gmt":"2022-04-01T20:12:34","slug":"cuccap-researchers-develop-new-methods-for-detection-and-analysis-of-downy-mildew-pathogen-on-cucumber","status":"publish","type":"post","link":"http:\/\/132.236.156.160\/cuccap\/2022\/01\/05\/cuccap-researchers-develop-new-methods-for-detection-and-analysis-of-downy-mildew-pathogen-on-cucumber\/","title":{"rendered":"CucCAP researchers develop new methods for detection and analysis of downy mildew pathogen on cucumber"},"content":{"rendered":"<p><img decoding=\"async\" loading=\"lazy\" class=\"size-full wp-image-10397 alignright\" src=\"http:\/\/132.236.156.160\/cuccap\/wp-content\/uploads\/sites\/6\/2021\/01\/cucumber-logo-min.jpg\" alt=\"cucumber illustration\" width=\"210\" height=\"229\" srcset=\"http:\/\/132.236.156.160\/cuccap\/wp-content\/uploads\/sites\/6\/2021\/01\/cucumber-logo-min.jpg 210w, http:\/\/132.236.156.160\/cuccap\/wp-content\/uploads\/sites\/6\/2021\/01\/cucumber-logo-min-138x150.jpg 138w\" sizes=\"(max-width: 210px) 100vw, 210px\" \/>The oomycete pathogen <em>Pseudoperonospora cubensis<\/em>, is a devastating foliar disease of cucumber. Growers seek to time initiation of their fungicide programs to the influx of the airborne <em>P. cubensis<\/em> sporangia into their production regions. However, the sporangia of <em>P. cubensis<\/em> captured by spore traps are visually indistinguishable from those of <em>P. humuli<\/em> (hop downy mildew) which often appear earlier in the season and could lead to unnecessary sprays. Researchers in Dr. Mary Hausbeck\u2019s group at Michigan State University have developed a new, highly sensitive DNA-based protocol to genetically distinguish the two pathogens from spore trap samples. The enhanced sensitivity also enables detection 5-10 days before symptoms appear in cucumber fields, providing a broader window for initiation of chemical control.<\/p>\n<hr \/>\n<p>For more information:<\/p>\n<ul>\n<li><strong><a href=\"https:\/\/apsjournals.apsnet.org\/doi\/10.1094\/PDIS-07-20-1534-RE\" target=\"_blank\" rel=\"noopener noreferrer\">Detection of Airborne Sporangia of <em>Pseudoperonospora cubensis<\/em> and <em>P. humuli<\/em> in Michigan using Burkard Spore Traps Coupled to Quantitative PCR<\/a><\/strong><br \/>\nby J.C. Rodriguez, M.L. Sakalidis, D. Perla, and M.K. Hausbeck.<br \/>\nPublished online by Plant Disease on <span class=\"epub-section__date\">April 9, 2021.<br \/>\n<\/span><span class=\"epub-section__item\"><span class=\"epub-section__state\">DOI: <\/span><\/span><span class=\"epub-section__item\"><a class=\"epub-section__doi__text\" href=\"https:\/\/doi.org\/10.1094\/PDIS-07-20-1534-RE\" target=\"_blank\" rel=\"noopener noreferrer\">10.1094\/PDIS-07-20-1534-RE<\/a><\/span><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Researchers in Dr. Mary Hausbeck\u2019s group at MSU have developed a highly sensitive DNA-based protocol to genetically distinguish between spores of cucumber downy mildew and hop downy mildew from spore trap samples. The enhanced sensitivity enables detection 5-10 days before symptoms appear in cucumber fields, providing a broader window for initiation of chemical control.<\/p>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"quote","meta":{"footnotes":""},"categories":[269,419],"tags":[472,400],"_links":{"self":[{"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/posts\/11888"}],"collection":[{"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/comments?post=11888"}],"version-history":[{"count":7,"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/posts\/11888\/revisions"}],"predecessor-version":[{"id":12441,"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/posts\/11888\/revisions\/12441"}],"wp:attachment":[{"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/media?parent=11888"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/categories?post=11888"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/132.236.156.160\/cuccap\/wp-json\/wp\/v2\/tags?post=11888"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}